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GFAP Antibody

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GFAP Antibody

  恒遠(yuǎn)生物抗體平臺現(xiàn)已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關(guān)抗體、小分子抗體、二抗、標(biāo)簽抗體及內(nèi)參抗體等。并以每年4000種的速度在持續(xù)增長。

  恒遠(yuǎn)生物擁有專業(yè)的質(zhì)控團隊,配備先進(jìn)實驗儀器,現(xiàn)已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標(biāo)記,大部分抗體都能進(jìn)行ELISA、WB、IHC、IF、IP、ChIP等應(yīng)用驗證。

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GFAP Antibody

 
  • 貨號:
    HYK-200116
  • 規(guī)格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
      All lanes: GFAP antibody at 3.9μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 50, 51 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA009369LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA009369LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

產(chǎn)品詳情

  • 產(chǎn)品名稱:
    Rabbit anti-Homo sapiens (Human) GFAP Polyclonal antibody
  • Uniprot No.:
    P14136
  • 基因名:
    GFAP
  • 別名:
    GFAP antibody; GFAP Epsilon antibody; GFAP_HUMAN antibody; GFAPdelta antibody; GFAPepsilon antibody; Glial fibrillary acidic protein antibody; Intermediate filament protein antibody
  • 宿主:
    Rabbit
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Recombinant Human Glial fibrillary acidic protein (292-432AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    >95%, Protein G purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IHC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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